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KMID : 0380620070390050558
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Korean Journal of Food Science and Technology
2007 Volume.39 No. 5 p.558 ~ p.563
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Inhibition of Gap Junctional Intercellular Communication in Rat Liver Epithelial Cells Induced by BHT and Propyl Gallate
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Kim Ji-Sun
Kim Sung-Ran
Ahn Ji-Yun
Ha Tae-Youl
Kang Kyoung-Sun
Kim Sun-A
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Abstract
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This study was conducted to analyze the cytotoxic effects of butylated hydroxytoluene (BHT) and propyl gallate (PG) in WB-F344 rat liver epithelial cells. Here we measured the inhibition level of gap junctional intercellular communication (GJIC) and elucidated the relationships between GJIC and mitogen-activated protein kinases (MAPKs) such as ERK, JNK, and p38. The cytotoxicities of BHT and PG appeared at concentrations of 1.0mM and 0.25mM, respectively, in the WB-F344 cells; and GJIC inhibition, which was analyzed by a scrape-loading/dye transfer assay and Western blotting analysis, appeared at 0.6mM for BHT and 0.1mM for PG, respectively. Also, the phosphorylations of Cx43, ERK, JNK, and p38 increased in dose-dependent manners. This suggests that BHT and PG treatments inhibited GJIC by the phosphorylation of MAPKs prior to cell damage.
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KEYWORD
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gap junctional intercellular communication, butylated hydroxy toluene, propyl gallate, MAPKs
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